Hidroxiesteroide deshidrogenasa

Stephen Kemp, MD, PhD  Former Professor, Department of Pediatrics, Section of Pediatric Endocrinology, University of Arkansas for Medical Sciences College of Medicine, Arkansas Children's Hospital

Stephen Kemp, MD, PhD is a member of the following medical societies: American Academy of Pediatrics , American Association of Clinical Endocrinologists , American Pediatric Society , Endocrine Society , Phi Beta Kappa , Southern Medical Association , Southern Society for Pediatric Research

Disclosure: Nothing to disclose.

The rat testis and liver or human liver microsomes were used for 11β-HSD1 assay. 11β-HSD1 activity was performed in the microsome according to a previously described method [17] . In brief, the assay tubes contained 25 nM substrate 11DHC (for rat) or cortisone (for human), spiked with 30,000 cpm their respective 3 H-11keto-steroid, mM NADPH and 5 mM glucose-6-phosphaare in the PBS buffer. 2 µg microsomes were added to each tube to initiate the reaction and the reaction mixture was incubated for up to 2 hrs, during which the reaction is within the linear range. The rest procedure was similar to 11β-HSD1 assay in intact cells.

Hidroxiesteroide deshidrogenasa

hidroxiesteroide deshidrogenasa

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